Properties of the Reverse Transcription Reaction in mRNA Quantification
نویسندگان
چکیده
منابع مشابه
Properties of the reverse transcription reaction in mRNA quantification.
BACKGROUND In most measurements of gene expression, mRNA is first reverse-transcribed into cDNA. We studied the reverse transcription reaction and its consequences for quantitative measurements of gene expression. METHODS We used SYBR green I-based quantitative real-time PCR (QPCR) to measure the properties of reverse transcription reaction for the beta-tubulin, glyceraldehyde-3-phosphate deh...
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ژورنال
عنوان ژورنال: Clinical Chemistry
سال: 2004
ISSN: 0009-9147,1530-8561
DOI: 10.1373/clinchem.2003.026161